Threshold is when a human female is forced to deal with any pokegirl blood in them. Not all girls with pokegirl ancestry go through Threshold, but those that do always do so during puberty. A female that succumbs to Threshold is forever changed into a pokegirl, but some researchers are trying to find a way to prevent Threshold or even reverse it. Threshold can happen anytime during puberty to a female, but is more likely to happen if they interact with pokegirls often. There are also some mod-chips for pokeballs that are said to force a human female to undergo Threshold when used.